Insertion junction: LMJ.RY0402.055323_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.055323
Chromosome:chromosome 3
Location:1376397
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g150800 (1 of 45) IPR029052 - Metallo-dependent phosphatase-like intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGCGTTGACTTGCGCTTATCTCCGTCCT
Internal bar code:GTCATCTTTTATTCAAGACCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:253
LEAP-Seq percent confirming:91.2029
LEAP-Seq n confirming:508
LEAP-Seq n nonconfirming:49
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TCAGACTGCTCCTTCCACCT
Suggested primer 2:AATTTCAGGACATGAACGGC