Insertion junction: LMJ.RY0402.055350_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.055350
Chromosome:chromosome 15
Location:214493
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre15.g635350 PRM4,PRMT4,CARM1 (1 of 1) K05931 - histone-arginine methyltransferase CARM1 [EC:2.1.1.125] (CARM1, PRMT4); Protein-/Histone-arginine N-methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGCAGCATGTGTCCGTGCCCCTTCCCGA
Internal bar code:ACGAAAAATAAATGGTTTTCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:917
LEAP-Seq percent confirming:98.9733
LEAP-Seq n confirming:482
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:AAGCTCTTGCCTTAAAGCCC
Suggested primer 2:CACTGAAGCCCCACACCTAT