| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.055361 |
| Chromosome: | chromosome 14 |
| Location: | 2736890 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g626950 | NIC1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCCCGACCGTCGACCGCCCCCTACTGGC |
| Internal bar code: | ACGACCCCCGGAAGGCGACGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 896 |
| LEAP-Seq percent confirming: | 99.592 |
| LEAP-Seq n confirming: | 2685 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTGGTTCAACAGCAGCAG |
| Suggested primer 2: | TCCTGGGGATGTGTATTGGT |