| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.055428 |
| Chromosome: | chromosome 12 |
| Location: | 3214372 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g498800 | (1 of 1) PTHR23176:SF0 - RHO GTPASE-ACTIVATING PROTEIN 68F | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTAGCCCTTTTGTCTATTTTTACGTTGAT |
| Internal bar code: | GCCATCTAGGTGCGGGCGGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 145 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 59 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGGAGATGGACACATGC |
| Suggested primer 2: | CAAACTTCGACGGACATCCT |