Insertion junction: LMJ.RY0402.055430_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.055430
Chromosome:chromosome 17
Location:5871243
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g739850 LRRC6,Seahorse,DNAAF11,MOT47 (1 of 8) PF14580 - Leucine-rich repeat (LRR_9); Leucine-rich repeat-containing protein 6 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCTAACCCTATCCCGCGCGTCGCTGCCT
Internal bar code:CCGAAAGTCTTCGCGAAGAGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:504
LEAP-Seq percent confirming:97.3958
LEAP-Seq n confirming:187
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:ACCTGACTTCGCGCTGTACT
Suggested primer 2:TGACAGCACTCACACAGCAG