| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.055442 |
| Chromosome: | chromosome 12 |
| Location: | 7540012 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g555650 | (1 of 30) PF03407 - Nucleotide-diphospho-sugar transferase (Nucleotid_trans) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGCGAAGGACTGATGAGCGTAGAGTCAC |
| Internal bar code: | CGGTGATGGTCGGCATAGGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 964 |
| LEAP-Seq percent confirming: | 99.4505 |
| LEAP-Seq n confirming: | 3801 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAGGGTGTGGTTTACGTC |
| Suggested primer 2: | CCTGGCTTAAGGGAGAGCTT |