Insertion junction: LMJ.RY0402.055454_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.055454
Chromosome:chromosome 2
Location:4966791
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g103950 MIA1,FAP100 (1 of 2) PF13863 - Domain of unknown function (DUF4200) (DUF4200); MIA1 subunit of the modifier of inner arms (MIA) complex CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCAAAGTCCAGGTTGGGCGGCTGCGGCTCC
Internal bar code:GCACCAGCTGCTTCGTGGCGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:515
LEAP-Seq percent confirming:99.7908
LEAP-Seq n confirming:477
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:ACCTTGTCTCCGAGCTCCTT
Suggested primer 2:TCAATGGCGGTCTAAACACA