| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.055454 |
| Chromosome: | chromosome_2 |
| Location: | 4966791 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g103950 | FAP100 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCAAAGTCCAGGTTGGGCGGCTGCGGCTCC |
| Internal bar code: | GCACCAGCTGCTTCGTGGCGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 515 |
| LEAP-Seq percent confirming: | 99.7908 |
| LEAP-Seq n confirming: | 477 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTTGTCTCCGAGCTCCTT |
| Suggested primer 2: | TCAATGGCGGTCTAAACACA |