| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.055463 |
| Chromosome: | chromosome_12 |
| Location: | 3271082 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g498200 | FAP128 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTCCGACCCCATTCCTGCTTGCCAACTTCA |
| Internal bar code: | GCTACTCTGGCTTCCCAGGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 995 |
| LEAP-Seq percent confirming: | 99.3172 |
| LEAP-Seq n confirming: | 5527 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTGGACATGTGAGGACG |
| Suggested primer 2: | GAGGGGACTGGAACTGTGAA |