Insertion junction: LMJ.RY0402.055495_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.055495
Chromosome:chromosome 6
Location:8182308
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g305350 (1 of 1) PTHR11319//PTHR11319:SF32 - G PROTEIN-COUPLED RECEPTOR-RELATED // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGGTCATAGGGAGGGATCGACCAGCAGGT
Internal bar code:CCCAGGAAGTCCCAGGTTTCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:232
LEAP-Seq percent confirming:99.6805
LEAP-Seq n confirming:936
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CCACTCTCACAAAAGGGCAT
Suggested primer 2:AAGGTTGGTGGTGAGAGTGG