| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.055511 |
| Chromosome: | chromosome 5 |
| Location: | 1912604 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241631 | (1 of 1) IPR000253//IPR002893 - Forkhead-associated (FHA) domain // Zinc finger, MYND-type | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGACCCTCCCCTGCTCGTCACCGACCCCT |
| Internal bar code: | CGGTAACAGGGGGGACGCTCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 991 |
| LEAP-Seq percent confirming: | 98.9796 |
| LEAP-Seq n confirming: | 97 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAGTCAGGGCTGAAGTGG |
| Suggested primer 2: | GCGGCCTCAGACTAGATACG |