| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.055554 |
| Chromosome: | chromosome 13 |
| Location: | 2585209 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g581050 | GT90F46,GT90-46 | (1 of 11) 2.4.2.26 - Protein xylosyltransferase / Uridine diphosphoxylose-protein xylosyltransferase; GT90 family protein 46 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCCTGATCGTATTGCCTTGCAGCAGGCG |
| Internal bar code: | CTTAGGCCGGGGGCTGCTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 594 |
| LEAP-Seq percent confirming: | 99.7876 |
| LEAP-Seq n confirming: | 1879 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTGTCGTCATAGGGCAGT |
| Suggested primer 2: | CACGTAGTGCACATTGGGTC |