| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.055558 |
| Chromosome: | chromosome 13 |
| Location: | 1068710 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g568750 | (1 of 1) K18667 - activating signal cointegrator complex subunit 2 (ASCC2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGTCACTGCCTCCGCCGCCGCCTCCGCC |
| Internal bar code: | GTTTTCGCTCTCCCCGGGCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 374 |
| LEAP-Seq percent confirming: | 99.3921 |
| LEAP-Seq n confirming: | 327 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGACGGCAAGATTTACAA |
| Suggested primer 2: | ACAAGTTGGGACCGTAGTGC |