Insertion junction: LMJ.RY0402.055563_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.055563
Chromosome:chromosome 7
Location:2875914
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g332300 GWD2,1 Alpha-glucan water dikinase 2; (1 of 3) 2.7.9.4 - Alpha-glucan, water dikinase / Starch-related R1 protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGCAGGTGAACTCATTGCTGTGTGCAACT
Internal bar code:TGTATTGTCGGAACAAGATCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:476
LEAP-Seq percent confirming:97.191
LEAP-Seq n confirming:173
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AGCCATCCTCTCCTCTCACA
Suggested primer 2:GCAGAGACACAACAGGCACA