Insertion junction: LMJ.RY0402.055569_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.055569
Chromosome:chromosome 3
Location:2482546
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g159600 (1 of 5) IPR008274 - Aldehyde oxidase/xanthine dehydrogenase, molybdopterin binding 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGACGCTATTCCTATACTAAGCAATAGTCA
Internal bar code:ACGCTCGGTGATGGCATTGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:372
LEAP-Seq percent confirming:95.5752
LEAP-Seq n confirming:108
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GTACGCAGGTTTCTTAGGCG
Suggested primer 2:GGACAGGTTTATGTTCGCGT