| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.055592 |
| Chromosome: | chromosome 16 |
| Location: | 609885 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g692150 | GT90-29,GT90F29 | (1 of 11) 2.4.2.26 - Protein xylosyltransferase / Uridine diphosphoxylose-protein xylosyltransferase; GT90 family protein 29 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAAAGCCGCATGTGAACTGGCATAGGTGC |
| Internal bar code: | ACTGGCGCTATGCTCCATATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 476 |
| LEAP-Seq percent confirming: | 99.7534 |
| LEAP-Seq n confirming: | 2832 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAGAGATGTACGCACTGA |
| Suggested primer 2: | GTCATGCGGTACAAGTCCCT |