| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.055610 |
| Chromosome: | chromosome_6 |
| Location: | 6640422 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g293950 | SHMT2 | Serine hydroxymethyltransferase 2 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATACATTTATCGTTAGACCCTTGTGACGGG |
| Internal bar code: | TTCAAATCTACTTGTGGCGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 928 |
| LEAP-Seq percent confirming: | 99.5996 |
| LEAP-Seq n confirming: | 5473 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCTGCTGGGAGGAGTTGT |
| Suggested primer 2: | GCGATACCCACCCTCTACAA |