| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.055611 |
| Chromosome: | chromosome_2 |
| Location: | 7857713 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g141800 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCTCAGGCGCTGCACCTGGTGCTGCTGCCG |
| Internal bar code: | GCGGAGGCCCTGTTCAATCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 129 |
| LEAP-Seq percent confirming: | 19.8014 |
| LEAP-Seq n confirming: | 5642 |
| LEAP-Seq n nonconfirming: | 22851 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCTCTGCTTTGTTGCCTC |
| Suggested primer 2: | TACTTAGTGGGCGGGATTTG |