| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.055622 |
| Chromosome: | chromosome 16 |
| Location: | 1336670 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g651801 | (1 of 7) IPR000104//IPR020683 - Antifreeze protein, type I // Ankyrin repeat-containing domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCCAGCCCCGGCAGCCAGGCGTAGTCAT |
| Internal bar code: | GGGCACAAGGCCTGTGACGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1050 |
| LEAP-Seq percent confirming: | 99.7308 |
| LEAP-Seq n confirming: | 6669 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGAAGATCGAGGAATGACA |
| Suggested primer 2: | TGGTACTGCTGTTGGTGGTG |