| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.055633 |
| Chromosome: | chromosome 4 |
| Location: | 3813823 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g229700 | UGP1,UGP | (1 of 1) K00963 - UTP--glucose-1-phosphate uridylyltransferase (UGP2, galU, galF); UDP-glucose pyrophosphorylase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCCTGCCAGCAAGGAGTCCACACCTTCC |
| Internal bar code: | ACGATGCGCATCGGGTAGGGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 589 |
| LEAP-Seq percent confirming: | 99.1828 |
| LEAP-Seq n confirming: | 2306 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGATTGTCTTATTCAGCA |
| Suggested primer 2: | GAGATTGGAAAGGAGGGGAG |