| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.055657 |
| Chromosome: | chromosome 10 |
| Location: | 825040 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g423450 | PPCDC1,COAC1, COAC1,COA4 | (1 of 1) 4.1.1.36 - Phosphopantothenoylcysteine decarboxylase / N-((R)-4'-phosphopantothenoyl)-L-cysteine carboxy-lyase; Phosphopantothenoylcysteine decarboxylase, CoA biosynthesis | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGCTGCTGCTTCACGCACCAAAGAACCT |
| Internal bar code: | GAGTATATTCATTGGTGCTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1263 |
| LEAP-Seq percent confirming: | 97.3278 |
| LEAP-Seq n confirming: | 7503 |
| LEAP-Seq n nonconfirming: | 206 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGTGTGTGCTCATGTGTG |
| Suggested primer 2: | TCGTCTACACAGCTCATCCG |