| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.055659 |
| Chromosome: | chromosome 12 |
| Location: | 1014871 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g491500 | UBA2,UBA3,UBC1 | RUB1/NEDD8 E1 activating enzyme; (1 of 1) K10686 - ubiquitin-activating enzyme E1 C (UBE1C, UBA3) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGGAGCGCAACAGCCGTATCCTGGGGAC |
| Internal bar code: | CCTTTACACATACGGACTGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 10 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 675 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAAAAGGTGTGCGAGTGT |
| Suggested primer 2: | ACAAGAGCGAGGATCAGGAA |