| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.055677 |
| Chromosome: | chromosome 15 |
| Location: | 534452 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g637100 | (1 of 1) K04523 - ubiquilin (UBQLN, DSK2); DSK2 homolog | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAAGTATCGAGGCCCTAGTGAAGTGATTG |
| Internal bar code: | GTGTCGGCAAGGCCGAAGCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1113 |
| LEAP-Seq percent confirming: | 99.6145 |
| LEAP-Seq n confirming: | 9819 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCCTTTTTGCACTCTCGC |
| Suggested primer 2: | GATCGGCAGTGTGACAGAGA |