| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.055714 |
| Chromosome: | chromosome 11 |
| Location: | 152876 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467544 | IDI1 | Isopentenyl-diphosphate delta-isomerase; (1 of 1) K01823 - isopentenyl-diphosphate delta-isomerase (idi, IDI) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCTGTTGATCACCGCCTCTTTCACCTGC |
| Internal bar code: | ACACAGTGCCGTGTTGGGGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 680 |
| LEAP-Seq percent confirming: | 99.5273 |
| LEAP-Seq n confirming: | 2737 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGTTGGAGCTCTCCTCAT |
| Suggested primer 2: | AATGTACTACCCCACGAGCG |