Insertion junction: LMJ.RY0402.055729_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.055729
Chromosome:chromosome_16
Location:1944517
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre16.g656300 DPR1 Dihydrodipicolinate reductase antisense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):CTTGGCCGCCCCGTTTATTTGTTTGTATAA
Internal bar code:CTTCGTCGCCTGGATTCTAACG

Confirmation - LEAP-Seq

LEAP-Seq distance:1409
LEAP-Seq percent confirming:99.5877
LEAP-Seq n confirming:2174
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GGTAGCCAAGTCGTGGATGT
Suggested primer 2:GTAAAGCGTAAGCAGCCAGG