Insertion junction: LMJ.RY0402.055754_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.055754
Chromosome:chromosome 2
Location:4215702
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g098700 (1 of 2) 3.6.3.19 - Maltose-transporting ATPase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGACGTGGCCGAACACCACCATGGGTATC
Internal bar code:CAGTACTTCGACCTGAACGGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:957
LEAP-Seq percent confirming:99.1912
LEAP-Seq n confirming:2085
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CCTGAACCTCATTGCCATCT
Suggested primer 2:TTGCCTTTGATACCCTGTCC