| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.055772 |
| Chromosome: | chromosome 10 |
| Location: | 5271942 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g457331 | (1 of 12) 2.7.13.3 - Histidine kinase / Protein kinase (histidine) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGCACGTGTTCGAAATCGATTTTGAGGC |
| Internal bar code: | ACCGCATGCAAACGAGCCAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1070 |
| LEAP-Seq percent confirming: | 97.6929 |
| LEAP-Seq n confirming: | 2456 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGACACGTTCTGTCCCTC |
| Suggested primer 2: | ACTACCGCACTGCCAGAGTT |