Insertion junction: LMJ.RY0402.055794_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.055794
Chromosome:chromosome 17
Location:744315
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre17.g701250 FLA12,PF8,CCDC39,FAP59 Flagellar Associated Protein 59; (1 of 1) PTHR18962//PTHR18962:SF0 - FAMILY NOT NAMED // COILED-COIL DOMAIN-CONTAINING PROTEIN 39 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCCCCTCCCTCGCTGCGTCCCTCTCCCT
Internal bar code:CGGTAAATCGCTTTCCCCCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:46
LEAP-Seq percent confirming:86.4078
LEAP-Seq n confirming:89
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TTGTAGGAGGCTGCGAAGTT
Suggested primer 2:AGCGTGTGGACACTAATCCC