Insertion junction: LMJ.RY0402.055801_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.055801
Chromosome:chromosome 7
Location:6279064
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g356850 (1 of 7) PF00582 - Universal stress protein family (Usp) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGCCTCGTAGCGGGTGTGCGCCATCAAT
Internal bar code:CTCCTTATTCGTGGTACTACA

Confirmation - LEAP-Seq

LEAP-Seq distance:784
LEAP-Seq percent confirming:99.4937
LEAP-Seq n confirming:393
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GGGTGCTTTCTGTTGTTGGT
Suggested primer 2:GCCCTGGACATACAGGAGAA