| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.055833 |
| Chromosome: | chromosome 1 |
| Location: | 769126 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g004000 | (1 of 1) PF08855 - Domain of unknown function (DUF1825) (DUF1825) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCCCTCCCCGCCCGTGGCCCCGCCGCAG |
| Internal bar code: | TGGCGCCCTGGCTTGATCTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 268 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTCTAGCATGCACGGAAA |
| Suggested primer 2: | CTCGGAACGTAGGTACTCGC |