Insertion junction: LMJ.RY0402.055841_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.055841
Chromosome:chromosome 3
Location:1386286
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g150800 (1 of 45) IPR029052 - Metallo-dependent phosphatase-like intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGAGCGGGGTCAGACACGCGACAGTCCCG
Internal bar code:ACCCTCCTCTCGTGTCAGGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:746
LEAP-Seq percent confirming:99.3531
LEAP-Seq n confirming:1843
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:TCAGCAGCATACTGACCCTG
Suggested primer 2:TCCACGCAAAACCATTGATA