| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.055844 |
| Chromosome: | chromosome 3 |
| Location: | 8180493 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g200250 | ICL3 | Putative isocitrate lyase/malate synthase; (1 of 1) 2.7.8.23 - Carboxyvinyl-carboxyphosphonate phosphorylmutase / CPEP phosphonomutase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCCCTGTTTGGTTTAGTTTGGGCTAGTA |
| Internal bar code: | ATAGTGTTCTCCAAATTCTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 38.4 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 77 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCCCTCAAACAACAGCTC |
| Suggested primer 2: | GGGAAGGGTACGTCAGACAA |