| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.055872 |
| Chromosome: | chromosome 16 |
| Location: | 3909425 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g690450 | FAP266 | Flagellar Associated Protein 266; (1 of 1) PTHR23084//PTHR23084:SF143 - PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCAAGTCCGCACCAGCACGCCATAAGCT |
| Internal bar code: | GTGCGGGTAGGCAGGCTCGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 887 |
| LEAP-Seq percent confirming: | 98.9036 |
| LEAP-Seq n confirming: | 14975 |
| LEAP-Seq n nonconfirming: | 166 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAGTTGTCACCACCCTCGC |
| Suggested primer 2: | ACTGGTTACAGCGGAGCACT |