Insertion junction: LMJ.RY0402.055884_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.055884
Chromosome:chromosome 11
Location:1048813
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g467672 (1 of 14) PTHR10334 - CYSTEINE-RICH SECRETORY PROTEIN-RELATED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGATTGATGTAACAAACGGACCTTCAATGA
Internal bar code:CCCCGCTATAGTTTCCACAACA

Confirmation - LEAP-Seq

LEAP-Seq distance:583
LEAP-Seq percent confirming:98.9899
LEAP-Seq n confirming:392
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CGAGGGGTGCTTTCATTTTA
Suggested primer 2:TGTGGAGGAGTGCTGTTGAG