| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.055895 |
| Chromosome: | chromosome 1 |
| Location: | 3954828 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g025750 | (1 of 1) PF01551 - Peptidase family M23 (Peptidase_M23) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGGTAAGGGCGCAAGCAGGTGGTGCGTG |
| Internal bar code: | GGCAGCTGTACCGGATCCTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1002 |
| LEAP-Seq percent confirming: | 99.6884 |
| LEAP-Seq n confirming: | 4159 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATATCCGCCGTTTGAAGTTG |
| Suggested primer 2: | ATGCCTGACACCTTACCGAC |