Insertion junction: LMJ.RY0402.055904_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.055904
Chromosome:chromosome 9
Location:2157190
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g393300 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGTGTGTGTGTGTGTGTGTGTTAGGAATC
Internal bar code:CGCCTCCGCGCTCCTTTCGATC

Confirmation - LEAP-Seq

LEAP-Seq distance:279
LEAP-Seq percent confirming:14.6657
LEAP-Seq n confirming:634
LEAP-Seq n nonconfirming:3689
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GGTTACAGCGTAATCCCGAA
Suggested primer 2:GCGTGTGTGTGTGTGTGTGT