| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.055908 |
| Chromosome: | chromosome 16 |
| Location: | 3565891 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g680588 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCCAGCGCCAGGGTCCCTGCTTTATTGC |
| Internal bar code: | GGCACTAGGTCCATTCTACACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 632 |
| LEAP-Seq percent confirming: | 65.9922 |
| LEAP-Seq n confirming: | 1696 |
| LEAP-Seq n nonconfirming: | 874 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAACTGGTGCAAAGTGCAG |
| Suggested primer 2: | AGATGTACAGCTGGGGGTTG |