| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.055941 |
| Chromosome: | chromosome 12 |
| Location: | 2407661 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g507200 | (1 of 2) IPR000104//IPR003613 - Antifreeze protein, type I // U box domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGTGGGCTCAGCTCACGTTTCCCTTGC |
| Internal bar code: | GTCCGACCCGGGTAGCGAGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 940 |
| LEAP-Seq percent confirming: | 98.45 |
| LEAP-Seq n confirming: | 1969 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGAGAGGCACTGGACTG |
| Suggested primer 2: | CAGTGGCGTTGCGTCTATAA |