| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.055964 |
| Chromosome: | chromosome 13 |
| Location: | 1851025 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g575333 | (1 of 1) PTHR11842//PTHR11842:SF10 - MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2 // MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2B | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGTGGTGGAGCGCGTGAGCTTCTGCACA |
| Internal bar code: | CGGGAGACTCCGCATGTAATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 346 |
| LEAP-Seq percent confirming: | 92.8889 |
| LEAP-Seq n confirming: | 209 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTAGCCACCCCCTACTA |
| Suggested primer 2: | ATGTAACATCGCGAAGACCC |