| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.055966 |
| Chromosome: | chromosome 16 |
| Location: | 7307522 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g684043 | ALDH24,ALDH24A1 | (1 of 1) 1.2.1.47 - 4-trimethylammoniobutyraldehyde dehydrogenase; Aldehyde dehydrogenase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGAGACGAACAAGACGAGTGTGTAACCT |
| Internal bar code: | AATATACTTCCGAGGCAACGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 613 |
| LEAP-Seq percent confirming: | 98.1203 |
| LEAP-Seq n confirming: | 522 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAGGCGCATATGGTAAGG |
| Suggested primer 2: | GGACAAGGTGGACACGTCTT |