| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.055979 |
| Chromosome: | chromosome 5 |
| Location: | 1639185 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g243150 | Carbonic anhydrase-related protein; (1 of 1) PF04357 - Family of unknown function (DUF490) (DUF490) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAATGTAACCCCCCTCCCCCTGCAGGTGCT |
| Internal bar code: | TACGTTCCACAGCCCGAGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 59 |
| LEAP-Seq percent confirming: | 64.6154 |
| LEAP-Seq n confirming: | 84 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGCAGTAGCAGCAACAA |
| Suggested primer 2: | CCTTGCTGAGAAGGGTGAAG |