| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.055989 |
| Chromosome: | chromosome 3 |
| Location: | 3659412 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g168900 | FMO12 | Flavin-containing monooxygenase; (1 of 1) PTHR23023:SF40 - DIMETHYLANILINE MONOOXYGENASE [N-OXIDE-FORMING] 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGCCCGTGGCCAGCACCACCAGCTGTGT |
| Internal bar code: | GGACCTTCGTTTTATGCGGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 456 |
| LEAP-Seq percent confirming: | 78.2502 |
| LEAP-Seq n confirming: | 3515 |
| LEAP-Seq n nonconfirming: | 977 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGGTGGTGGGTGATGATA |
| Suggested primer 2: | ATAGTGCCGTGGACCTAACG |