| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.055993 |
| Chromosome: | chromosome 3 |
| Location: | 5222108 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g182800 | AGT2 | Alanine-glyoxylate transaminase; (1 of 1) K00827 - alanine-glyoxylate transaminase / (R)-3-amino-2-methylpropionate-pyruvate transaminase (AGXT2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGATACTCTTGCAGGGTGTTGTGCAGC |
| Internal bar code: | CTGGGACACGCGGGTTCAGCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 173 |
| LEAP-Seq percent confirming: | 47.4741 |
| LEAP-Seq n confirming: | 2885 |
| LEAP-Seq n nonconfirming: | 3192 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTGCAGACTCCAAGGTCA |
| Suggested primer 2: | GCATCCCTCACATCCCTAGA |