| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.055994 |
| Chromosome: | chromosome 6 |
| Location: | 5038084 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g280350 | (1 of 5) IPR001680//IPR011047//IPR017986//IPR020472 - WD40 repeat // Quinonprotein alcohol dehydrogenase-like superfamily // WD40-repeat-containing domain // G-protein beta WD-40 repeat | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGACTGGCGCTGGAGGAGCAGCAGGGCAG |
| Internal bar code: | TCCGTCACTCCCGGTGGATGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 397 |
| LEAP-Seq percent confirming: | 99.2627 |
| LEAP-Seq n confirming: | 1481 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATCGTTAACTTCGCCTTC |
| Suggested primer 2: | AGGGAGAGATGCTGTGGCTA |