| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.056033 |
| Chromosome: | chromosome 12 |
| Location: | 8656541 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g546750 | Putative peroxisomal membrane protein; (1 of 34) IPR000571 - Zinc finger, CCCH-type | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAATCGTTCCTTTACAACACGGGCATGG |
| Internal bar code: | TCTCTCTGCGATGCTCAATAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 104 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 92 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTTCATAAGTGGGGCTGG |
| Suggested primer 2: | AATACGGAGACACGAAACCG |