| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.056041 |
| Chromosome: | chromosome 13 |
| Location: | 1575099 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g573150 | FPN5 | Purine 5'-nucleotidase; (1 of 1) PTHR12103:SF15 - PROTEIN Y71H10B.1, ISOFORM C | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACTACAACATGTCGCTGTACGAGGTAGG |
| Internal bar code: | TGTGCGCCCACAATATGTAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 584 |
| LEAP-Seq percent confirming: | 98.8908 |
| LEAP-Seq n confirming: | 7935 |
| LEAP-Seq n nonconfirming: | 89 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAATGGTCGTGGGAGTGAG |
| Suggested primer 2: | ATCAGTTGTGCCTGAATCCC |