| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.056047 |
| Chromosome: | chromosome 9 |
| Location: | 5819963 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402145 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGATGCCGGCTATCGTGTCGGGCCCTCCT |
| Internal bar code: | CAGGGGGCATGGTTGCAAACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 603 |
| LEAP-Seq percent confirming: | 35.4712 |
| LEAP-Seq n confirming: | 2345 |
| LEAP-Seq n nonconfirming: | 4266 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGGTGTCGTTTTTGATTC |
| Suggested primer 2: | GTTACGCACCATTGTGGTTG |