Insertion junction: LMJ.RY0402.056051_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.056051
Chromosome:chromosome 2
Location:1411581
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g083400 (1 of 1) PF07000 - Protein of unknown function (DUF1308) (DUF1308) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCGCCAGCTGGTCGCGCCCCAGGCACTCG
Internal bar code:GGGACGTCCGGAAGCGGTTCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:218
LEAP-Seq percent confirming:99.4949
LEAP-Seq n confirming:197
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GGTCGCAAGAGTCAAGGAAG
Suggested primer 2:CTCCCTTTCTCCAACCCTTC