| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.056051 |
| Chromosome: | chromosome 2 |
| Location: | 1411584 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g083400 | (1 of 1) PF07000 - Protein of unknown function (DUF1308) (DUF1308) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGCTGGGCGACGGGCGCAGAGCGGTGCG |
| Internal bar code: | AGTTATTGAGCAGGATAAAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 485 |
| LEAP-Seq percent confirming: | 98.1818 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCGCAAGAGTCAAGGAAG |
| Suggested primer 2: | CTCCCTTTCTCCAACCCTTC |