| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.056060 |
| Chromosome: | chromosome 11 |
| Location: | 3275574 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g480050 | EXN14 | Exoribonuclease with BTB/POZ domain; (1 of 8) PTHR26379:SF144 - BTB/POZ DOMAIN-CONTAINING PROTEIN 19 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGTGAAGTTGAACTTGGTGGGTGGCGGTG |
| Internal bar code: | CCTGTCCGTCACTGGGGAGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 283 |
| LEAP-Seq percent confirming: | 98.1028 |
| LEAP-Seq n confirming: | 3568 |
| LEAP-Seq n nonconfirming: | 69 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACTGATGGTGAGGTCAGA |
| Suggested primer 2: | CTCGTGTTTGCGTGTTAGGA |