| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.056108 |
| Chromosome: | chromosome 7 |
| Location: | 1146570 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g320500 | OPR29 | OctotricoPeptide Repeat protein 29 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGCTTATGCCTCGCGATTTCGTCAATAG |
| Internal bar code: | GACCGATGGAGATCCACGCTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 492 |
| LEAP-Seq percent confirming: | 65.8188 |
| LEAP-Seq n confirming: | 414 |
| LEAP-Seq n nonconfirming: | 215 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTATAGGCAAGACGGTCA |
| Suggested primer 2: | CGCTCCAGATCCTGCTTTAC |